The role of a Graphical User Interface (GUI) in software applications is to provide users with an intuitive visual way to interact with the software. A GUI allows users to engage with programs through elements like menus, buttons, and icons instead of relying solely on command-line inputs. This enhances user experience by making software more accessible and user-friendly, reducing the learning curve for new users. GUIs are essential for improving overall productivity and satisfaction, especially in complex applications where ease of navigation is crucial.