The difference between an organism and its environment lies in their respective roles within an ecosystem. An organism refers to any individual living entity, including plants, animals, and microorganisms, that can perform vital life processes. In contrast, the environment encompasses all external factors that influence the survival and behavior of organisms. These factors include abiotic elements like temperature and water, and biotic elements such as interactions with other organisms. The interplay between organisms and their environment shapes ecological dynamics and drives evolutionary changes.