The significance of the separation of powers in U.S. politics lies in its designed prevention of tyranny, ensuring that no single branch of government gains overwhelming authority. By dividing the government into three branches—executive, legislative, and judicial—individual liberties are protected through a system of checks and balances. This framework promotes accountability and fosters cooperation among branches while allowing for effective governance. It also enhances political stability by enabling the resolution of conflicts through law rather than military or unilateral action.