The significance of the Labyrinth in Greek mythology is primarily related to the Minotaur, a creature that symbolizes chaos and the beast within. Designed by Daedalus, the Labyrinth was a complex maze where the Minotaur dwelled, requiring heroes like Theseus to confront their fears and emerge victorious. It represents the struggle between civilization and primitive instincts, as well as bravery in the face of adversity.