The river that flows through Rome is the Tiber River. This ancient river has significant historical and cultural importance, as it played a crucial role in the development of the city of Rome and the Roman Empire. The Tiber spans approximately 406 kilometers and is the third-longest river in Italy. It served as a key trade and transport route, while itsbanks host numerous historical landmarks, including the Vatican City. The Tiber also connects to the Mediterranean Sea, influencing commerce and culture in ancient times.